pegRNA-BLAST · prime-editing C-terminal knock-in designer

Finds pegRNAs for C-terminal tagging (recoded TJ-PE, neurogenomics/prime-editing). Fill in the template and cargo, choose parameters, and click Design pegRNA. fake = design logic with stand-in scorers; api = real gates (CPU + hosted-model APIs).

Design parameters

Target template
Cargo (insert)
    Genomic context & pegRNA parameters
    Advanced — full config JSON (authoritative on submit if edited)
    Edit here for keys not shown above (het_snps, manifest, _comment…). Click “Sync ← fields” to regenerate from the form.

    Processing pipeline

    The recoded TJ-PE design flow. Run a design to light up which stage each candidate reaches.
    ✓ passed ✕ blocked ○ not reached

    Result

    Load the example or fill the form, then Run design. Output appears here.